Intranucleolar localization of DNA topoisomerase IIalpha is a distinctive feature of necrotic, but not of apoptotic, Jurkat T-cells

Two distinct types of cell death have been described: apoptosis and necrosis. However, it is becoming increasingly clear that the differences between these two types are far less numerous than initially thought. Morphological analyses might provide important information to distinguish apoptotic from necrotic samples. We recently reported that in necrotic, but not apoptotic, HL-60 human myeloid leukaemia cells, the nuclear protein topoisomerase IIalpha concentrated in nucleoli. In order to ascertain whether or not this phenomenon was restricted to a peculiar cell type or could be detected also in cells of lymphoid lineage, we performed an investigation aimed at defining the localization of topoisomerase IIalpha in apoptotic and necrotic Jurkat human T lymphoblastoid cells. Immunofluorescence staining demonstrated that topoisomerase IIalpha was excluded from the condensed chromatin of apoptotic cells, whereas in necrotic cells it was localized in discrete nuclear dots. Immuno-electron microscopy analysis showed that topoisomerase IIalpha was undetectable in nucleoli of normal and apoptotic cells, whereas it was present in the nucleolus of necrotic cells irrespectively of the type of inducer used (ethanol, H(2)O(2), HgCl(2)). Taken together, our findings identify topoisomerase IIalpha as a potential morphological marker useful to discriminate between apoptotic and necrotic cells.     

Distinguishability quantification of fuzzy sets

Distinguishability is a semantic property of fuzzy sets that has a great relevance in the design of interpretable fuzzy models. Distinguishability has been mathematically defined through different measures, which are addressed in this paper. Special emphasis is given to similarity, which exhibits sound theoretical properties but its calculation is usually computationally intensive, and possibility, whose calculation can be very efficient but it does not exhibit the same properties of similarity. It is shown that under mild conditions - usually met in interpretable fuzzy modeling - possibility can be used as a valid measure for assessing distinguishability, thus overcoming the computational inefficiencies of similarity measures. Moreover, procedures that minimize possibility also minimize similarity and, consequently, improve distinguishability. In this sense, the use of possibility is fully justified in interpretable fuzzy modeling.     

Spectral estimation by least-squares optimization based on rational covariance extension

This paper proposes a new spectral estimation technique based on rational covariance extension with degree constraint. The technique finds a rational spectral density function that approximates given spectral density data under constraint on a covariance sequence. Spectral density approximation problems are formulated as nonconvex optimization problems with respect to a Schur polynomial. To formulate the approximation problems, the least-squares sum is considered as a distance. Properties of optimization problems and numerical algorithms to solve them are explained. Numerical examples illustrate how the methods discussed in this paper are useful in stochastic model reduction and stochastic process modeling.     

Can newborns discriminate between their own cry and the cry of another newborn infant?

Two experiments were conducted to test whether newborns could discriminate between their own cry and the cry of another newborn infant. Facial behavior and nonnutritive sucking rate were adopted as dependent measures. In Experiment 1, 20 newborns in an awake state were presented with either their own cry or the cry of another infant. In the latter condition, newborns showed the facial expression of distress more frequently and for a longer duration. In addition, the rate of sucking decreased significantly between the pretest phase and the 1st min of presentation of another infant's cry. Newborns' responses, although delayed and less intense, showed a similar trend in Experiment 2, during which 20 newborns in a sleep state were tested with the same procedure. These results indicate the newborns' capability to discriminate between the 2 cry stimuli and show the effectiveness of a newborn cry in inducing distress signals in another newborn infant. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Synthesis of 8-hydroxyquinoline functionalised DO3A ligand and Eu(III) and Er(III) complexes: Luminescence properties

The synthesis of a new DO3A-based macrocyclic ligand bearing a 8-hydroxyquinoline residue together with the preparation of its Eu³⁺ and Er³⁺ neutral complexes are described. In a previous report [F. Rizzo, A. Papagni, F. Meinardi, R. Tubino, M. Ottonelli, G.F. Musso, G. Dellepiane, Synth. Met. 147 (2004) 143], we have shown that lanthanide complexes display very high stability combined with a good luminescence in aqueous solution under UV radiation, which indicate an energy transfer process from the excited 8-hydroxyquinoline moiety to the metal. In this work, we correlate the ability to transfer the energy from the sensitizer to lanthanide ion with pH behaviour of the antenna. Furthermore, the variation of pH in Eu³⁺ complex supports the hypothesis of presence of charge-transfer transitions. The good solubility and sensitized emission in different solvents (organic and water) are very important aspects for their technologic applications as luminescent probes or NIR-emitting devices.     

Fluorinated membranes as interfaces for application in catalysis

In this work the use of poly(vinylidene) fluoride based membrane as interfaces for the biphasic oxidation of benzyl alcohol to benzaldheyde has been reported. The membranes have been prepared by means of dry-wet phase inversion. Membranes with different surface characteristics have been selected for the oxidation reaction.     

Ketoprofen Spray-dried Microspheres Based on Eudragit® RS and RL: Study of the Manufacturing Parameters

The preparation of ketoprofen spray-dried microspheres can be affected by the long drug recrystallization time. Polymer type and drug–polymer ratio as well as manufacturing parameters affect the preparation. The purpose of this work was to evaluate the possibility to obtain ketoprofen spray-dried microspheres using the Eudragit® RS and RL; the influence of the spray-drying parameters on morphology, dimension, and physical stability of microspheres was studied. Ketoprofen microspheres based on Eudragit® blend can be prepared by spray-drying and the nebulization parameters do not influence significantly particle properties; nevertheless, they can be affected by drying and storage methods. No effect of the container material is found.     

Identification of new epidemiological molecular markers by comparative proteomics of serogroup A meningococcal isolates from three pandemic waves

We previously described the first reference map for the proteome of one strain of serogroup A Neisseria meningitidis (MenA), a major cause of epidemic meningitis in humans. As a preliminary finding, in that work we noted that 2‐DE protein maps of closely related MenA isolates from different epidemics spreads could be easily compared to detect minor differences and that 2‐DE phenotypes attributable to the well‐known epidemiological marker tbpB agreed with the genoclouds model of MenA epidemiological variation during pandemic waves. We explored here the possibility that an extended comparative study of 2‐DE maps of isolates representative of the nine genoclouds described by Achtman and collaborators could be used to discriminate between strains otherwise undistinguishable. We showed the example of 14 proteins with different 2‐DE spot patterns in different genoclouds that could be considered as putative tracers for alike‐strains discrimination. We introduce the novel concept that comparative proteomics can be useful in identifying new epidemiological markers for N. meningitidis.     

Liquid-crystallization induced reactions. Microstructure and morphology of copolyesters synthesized by transesterification of PET with some LCP monomers

The transesterification of poly(ethylene terephthalate) (PET) with a mixture of sebacic acid (S), 4,4′-diacetoxybiphenyl (B) and 4-acetoxybenzoic acid (H), carried out under conditions expectedly favoring the formation of a p(ET-SBH) random copolyester, produces biphasic materials with an isotropic matrix and a highly fibrous, liquid-crystalline dispersed phase. Spectroscopic, calorimetric, microscopic and diffractometric characterization of the fractions separated by solvent extraction has shown that the two phases consist of practically random copolyesters having different average composition. Interestingly, the degree of aromaticity of the matrix is even lower than that of PET, whereas that of the minor phase is appreciably higher than that calculated for the SBH copolyester that would be produced from the monomer mixture in the absence of PET. This unexpected result is interpreted on the basis of an enthalpy-driven progressive diffusion of aromatic-rich material toward the mesophase which segregates at an early stage of the polycondensation within the isotropic mixture of low molar mass oligomers initially produced by the PET acidolysis. Thus, an increasing differentiation, rather than an equilibration, of the composition of the two phases takes place. It is noteworthy that, despite the strong compositional difference, the two phases of these products show fairly good compatibility and interfacial adhesion.